Product Information
Brand
Strata-X Solid Phase Extraction
Strata-X solid phase extraction (SPE) polymeric SPE sorbents provide extremely high recoveries and superior sample clean up. Strata_X SPE sorbents are available in the following formats SPE Tubes, 96-well plates, online SPE, 96-well microeluton plates, and Giga tubes
Strata-X Solid Phase Extraction
Robust, Accurate Polymeric Solid Phase Extraction (SPE) Products

Capitalize on cleanliness without sacrificing recovery with Strata-X polymeric SPE sorbents for solid phase extraction. Strata-X polymeric sorbents are available in unique selectivities that have been developed to cover a diverse spectrum of analytes and simplify the method development process for fast and efficient sample preparation.

  • Tightly retain analytes
  • Remove unwanted contaminants including phospholipids, proteins, and particulates for superior chromatography
  • Easily cleanup large or small volumes
  • Strata-XL cleans up viscous samples as well as target analytes that exceed 10 kDa
  • The 96-well plate microelution Strata-X formats are ideal for concentrated extraction for small volumes


Strata-X Solid Phase Extraction
Available Formats
96-Well Plates
Microelution 96-Well Plates
1, 3, and 6 mL Tubes
Giga Tubes (12-150 mL Tubes)
On-line Extraction Columns and Cartridges

Identify the SPE Retention Mechanism

Identify the Specific SPE Retention Mechanism

SPE
Mechanism
Analyte
Functional Group
Sorbent
Functional Group
Strata-X Sorbent
Reversed Phase
R
hydrocarbon

aromatic
R
hydrocarbon

aromatic
X, XL
Ion-Exchange
NR4+ strong
RNH3+ weak
RSO3- strong
RCO2- weak
-O2C—weak
-O3S—strong
+H3N—weak
+R3N—strong
X-CW, XL-CW
X-C
X-AW, XL-AW
X-A, XL-A

Select Your Particle and Pore Size

Sample Matrix Strata-X, X-C, X-A, X-CW, X-AW Strata-XL, XL-C, XL-A, XL-CW, XL-AW
Particle & Pore Size
33 μm, 85 Å
100 μm, 300 Å
High Concentration Samples
 
Small Target Analytes (< 10 kDa)
 
Large Target Analytes (> 10 kDa)
 
Large Volume Samples
 
Viscous Samples
 

Loading Capacities

Sample Matrix Sorbent Mass Strata-X,
X-C, X-CW, X-A, X-AW
Strata-XL,
XL-C, XL-CW, XL-A, XL-AW
Blood, serum, plasma
30 mg 250 µL 125 µL
Urine
30 mg 1 mL 500 µL
Filtered tissue homogenates
60 mg 100 mg 50 mg
Oral Fluid
30 mg 500 mg 250 mg


Environmental Samples Sorbent Mass Strata-X,
X-C, X-CW, X-A, X-AW
Strata-XL,
XL-C, XL-CW, XL-A, XL-AW
Water (particulate-free) drinking
200 mg 100 - 400 mL 50 - 200 mL
Water (particulate-laden) rivers, runoff, etc.
500 mg 100 - 400 mL 50 - 200 mL
Soil extracts
500 mg 100 g 50 g
Food/Plant Material
100 mg 10 mg 500 mg

Sorbent Wash and Elution Volumes*

The volume of solvent needed for the wash and elution steps is directly related to the mass of sorbent in the SPE tube and more specifically the “bed volume” of the SPE device. Typically 4 – 16 bed volumes are used in SPE methods.

Sorbent Mass 2 mg 10 mg 50 mg 100 mg 150 mg 200 mg 500 mg 1 g 2 g 5 g 10 g
Practical Minimum Wash and Elution Volume 4 bed volumes 200 µL 60 µL 300 µL 600 µL 900 µL 1.2 mL 3 mL 6 mL 12 mL 30 mL 60 mL
Recommended Wash and Elution Volume 8 bed volumes 50 µL 120 µL 600 µL 1.2 mL 1.8 mL 2.4 mL 6 mL 12 mL 24 mL 60 mL 120 mL
*The elution volumes are specific to the chemical nature of the analyte being extracted, its concentration in the sample, the chemical nature of the eluting solvent and the bed mass used. The above is a guideline. An elution study should be conducted to determine the appropriate volume to use.

Polymeric SPE Options

The volume of solvent needed for the wash and elution steps is directly related to the mass of sorbent in the SPE tube and more specifically the “bed volume” of the SPE device. Typically 4 – 16 bed volumes are used in SPE methods.

* Dry cartridge 5 - 10 mins prior to elution to remove residual solvents.