Product Information
Phase Detail
Luna C18(2) HPLC Columns
Luna C18(2) HPLC column phase is a very hydrophobic pH stable (1-10) C18 column that offers great performance for USP methods and general reversed-phase C18 applications
Luna C18(2) HPLC Columns

The Luna brand of columns and media is more than just a product line from Phenomenex. It is a pledge to provide you with the highest level of satisfaction for your chromatographic goals. Luna C18(2) phase is an octadecyl silane with ligands bound to the silica surface,resulting in a C18 HPLC column with very hydrophobic stationary phase that offers great methylene selectivity

  • Extensive line of rugged particle sizes
  • Easy method scalability from HPLC to preparative and bulk purification LC
  • C18(2) Ultra pure, metal-free silica (99.99% purity) (USP:L1)





Carbon Load
17.5
Particle Size
10,15,2.5,3,5
Surface Area
400
Recommended Use
c18(2).RecommendedUse
Long Column Lifetimes and Excellent Performance
Ultra-pure, metal-free silica (99.99 % purity) is the back bone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.

Superior Particle Smoothness

Agilent Technologies® ZORBAX® 5 µm SB-C18
Waters® Symmetry® 5 µm C18
Phenomenex Luna 5 µm C18
The high efficiencies and bonded phase surface coverage provide for sharp peaks The C8 columns are generally better hydrogen bond acceptors, and better for acidic compounds.

Peak Asymmetry Comparison

Peak Asymmetry Comparison
Conditions for all columns:

Dimension:
150 x 4.6 mm
Mobile Phase:
Acetonitrile/Water (50:50)
Flow Rate:
1.0 mL/min
Temperature:
Ambient
Detection:
UV @ 254 nm
Sample:
1. Pyridine
2. Phenol
3. Toluene

The high efficiencies and bonded phase surface coverage provide for sharp peaks Whether you need a column for USP methods or just general method development the Luna C18(2) should be your first choice every time.

Polar, Acidic Drugs

Polar Acidic Drugs Comparison
Conditions for all columns:

Columns:
Phenomenex Luna 3 µm C18(2)
Waters® Symmetry® 3.5 µm C18
Dimension:
75 x 4.6 mm
Mobile Phase:
20 mM KH2PO4/ Acetonitrile (70:30)
Flow Rate:
0.75 mL/min
Detection:
UV @ 202 nm
Sample:
1. Tolmetin
2. Naproxen
3. Diflunisal
4. Fenoprofen
5. Indomethacin
6. Ibuprofen



Hydrophobic, Acidic Compounds

Hydrophobic, Acidic Compounds Comparison
Conditions for all columns:

Columns:
Phenomenex Luna® 5 µm C18(2)
Thermo Hypersil-Keystone® HyPURITY™ Elite 5 µm C18
Dimension:
150 x 4.6 mm
Mobile Phase:
20 mM Potassium phosphate, pH 2.5 / Acetonitrile (75:25)
Flow Rate:
1.5 mL/min
Temperature:
30 °C
Detection:
UV @ 254 nm
Sample:
1. p-Hydroxybenzoic acid
2. Sorbic acid*
3. Benzoic acid*
4. Salicylic acid
5. p-Toluic acid
* Sorbic acid and Benzoic acid co-elute on HyPURITY Elite

Basic Compounds

Basic Compounds Comparison
Comparative separations may not be representative of all applications.
Conditions for all columns:

Columns:
Phenomenex Luna® 5 µm C18(2)
Macherey-Nagel® Nucleosil® 5 µm C18
Dimension:
150 x 4.6 mm
Mobile Phase:
20 mM Potassium phosphate, pH 2.5/Acetonitrile (75:25)
Flow Rate:
1.5 mL/min
Temperature:
30 °C
Detection:
UV @ 210 nm
Sample:
1. Maleic acid
2. Triprolidine*
3. Chlorpheniramine*
4. Diphenhydramine*
*Peaks 2-4 adsorb on Nucleosil C18