Product Information
Phase Detail
Kinetex Core-Shell Biphenyl HPLC Columns
Kinetex core-shell biphenyl HPLC columns offer increased separation power for polar and non-polar analytes
Kinetex Core-Shell Biphenyl HPLC Columns
Enhanced Polar and Aromatic Selectivity

Kinetex Biphenyl is a high-efficiency core-shell product capable of adding extra separation power to your analysis of non-polar and polar compounds. Take advantage of multiple selectivity interaction mechanisms such as hydrophobic, pi-pi, and dipole-dipole to improve tough separations.

  • Compatible with All Analytical HPLC/UHPLC Systems*
  • Orthogonal Selectivity to C18 Phase
  • 100 % Aqueous Mobile Phases Stable
  • USP: L11

*Kinetex analytical columns are compatible with all LC system with standard LC column end-fittings threaded in 10-32. Please contact us at Phenomenex.com/chat for any questions and information about fitting compatibility and any other column internal diameters.

knx_biphenyl

Particle Size
1.7,2.6,5
Recommended Use
100 % aqueous stable reversed phase chemistry with hydrophobic, aromatic, and enhanced polar selectivity
Novel Selectivity For Tough Separations

Think high performance‚ enhanced retention‚ and the ability to go where a traditional C18 can’t. The Kinetex Biphenyl offers the high performance benefits of a core-shell particle with a unique stationary phase capable of becoming the go-to selectivity for reversed phase method development. Use Kinetex Biphenyl columns to get enhanced retention‚ higher sensitivity‚ and overall better results;
especially for aromatic compounds.

hydrogen_bond
Aromatic Selectivity
Compounds with aromatic ring structures offer a specific type of selectivity associated with pi-pi bond interaction. The compound’s aromaticity provides pi electrons that have the potential to interact with pi bonds, which can be found on phenyl based stationary phases. This provides a unique, orthogonal selectivity compared to a traditional C18 phase.

Conditions for all columns:

Column:
Kinetex 1.7 µm Biphenyl
Dimension:
50 x 2.1 mm
Part No.:
00B-4628-AN
Mobile Phase:
A: Water with 0.1 % Formic Acid
B: Acetonitrile with 0.1 % Formic Acid
Gradient:
Time (min)
0
4
5
7
 
% B
5
100
100
5
Flow Rate:
0.5 mL/min
Temperature:
40 ˚C
Detection:
MS/MS (Sciex® API 4000™) (ambient)
Injection Volume:
10 μL
Sample:
Drugs of Abuse

Increased Separation of Aromatic Compounds
Using a Biphenyl Phase
The elution order of analytes can change depending upon the predominate selectivity characteristics of a HPLC or UHPLC column and the utilized mobile phase. Therefore, by altering the stationary phase or mobile phase conditions, we can observe a unique elution order and extend retention of more polar analytes.

Aromatic Compound Comparison

Conditions for all columns:

Columns:
Kinetex 1.7 µm C18
Kinetex 1.7 µm Biphenyl
Dimension:
50 x 2.1 mm
Part No.:
00B-4628-AN
00B-4475-AN
Mobile Phase:
A: Water with 0.1 % Formic Acid
B: Methanol with 0.1 % Formic Acid
Gradient:
Time (min)
0
4
4.1
 
% B
10
100
10
Flow Rate:
400 µL/min
Temperature:
50 ˚C
Detection:
MS/MS (Sciex API 4000™) (ambient)
Injection Volume:
10 µL
Backpressure:
450 Bar
Sample:
1. Morphine
2. Hydromorphone