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Clarity LC Synthetic Oligonucleotide Purification HPLC Columns: Phenomenex
Clarity LC reversed phase HPLC columns provide effective purification for synthetic oligonucleotides. Clarity HPLC columns are available in C18 selectivities and core-shell particles for optimized efficiency.
Clarity HPLC/PREP Columns

Each product in the Clarity BioSolutions portfolio has been designed to efficiently and effectively purify or characterize synthetic oligonucleotides used in biological research, therapeutic development and biochemical manufacturing.

  • RPC, HPLC, PREP LC, desalting, and extraction solutions
  • DNA, RNA/RNAi, longmers, dye-labeled, and modified oligonucleotides
  • High efficiency LC-MS protocols for characterization/QC
  • Personalized technical support and customer service

HPLC Column
LC-MS Analysis

Oligo-XT

An increased need for TK and PK/PD studies for oligo therapeutics has lead to an increase in LC-MS/MS analysis because of the specificity, sensitivity, and shorter method development times as compared to ligand binding assays or ELISA. Because sensitivity is of the utmost importance, it is crucial to select an LC column that provides both separation and high efficiencies to ensure accurate results. 

Clarity Core-Shell


Conventional Fully Porous

The Core-Shell Advantage

Unlike traditional fully porous oligo columns, Clarity Oligo-XT relies on the power of core-shell technology to provide extremely high efficiencies for both low and high oligo concentrations. Because the Clarity Oligo-XT particle is not fully porous, analytes spend less time diffusing into and out of the pores as they travel through the column, resulting in less band broadening for higher peak efficiencies.

Fully
Porous
  Clarity
Core-Shell
Average Efficiency
Gain with Clarity*
VS.

90 % Higher

VS.

85 % Higher

VS.

20 % Higher

Clarity Oligo-XT is patented by Phenomenex. U.S. Patent No. 7,563,367 and 8,658,038 and foreign counterparts.
*May not be representative of all applications

Analytical LC

Clarity Oligo-RP™

Clarity Oligo-RP columns have been specifically designed for the reversed phase purification of oligonucleotides with balanced hydrophobicity and polar selectivity. Available in 3, 5, and 10 µm particles, Clarity Oligo-RP allows for high capacity analysis and purification of oligos.

A Highly Selective Analysis

Clarity Oligo-RP successfully separates a 40mer from a 39mer DNA oligonucleotide due to its excellent efficiency and resolving power.
Separation of failure N-1 from target N sequence

Column:
Clarity 3 µm Oligo-RP C18
Dimensions:
50 x 4.6 mm
Part No.:
00B-4441-E0
Mobile Phase:
A: 50 mM TEAA pH 7.5
B: Methanol
Gradient:
10 % to 45 % B in 30 minutes
Flow Rate:
1 mL/ min
Detection:
UV @ 260 nm
Sample:
1. 40nt DNA with sequence
CTTCTGAACAGTTGATCTATG-
CACTTCAGACTTATGATCA (2.5 μg)
2. 39nt DNA with sequence
TTCTGAACAGTTGATCTATGCAC-
TTCAGACTTATGATCA (2.5 μg)

Purification

DNA Purification

Clarity Oligo-RP columns allow for high loadability and deliver high recovery and purity, from HPLC to Preparative Purification.

Easily Scale from HPLC to Preparative Purification

Column:
Clarity 3 µm Oligo-RP C18
Dimensions:
A: 50 x 10.0 mm
B: 50 x 4.6 mm
Part No.:
A: 00B-4441-N0
B: 00B-4441-E0
Mobile Phase:
A: 50 mM TEAA pH 7.5
B: Methanol
Gradient:
10 % to 60 % B in 20 minutes
Flow Rate:
A: 4.7 mL/ min
B: 1.0 mL/ min
Detection:
UV @ 260 nm
Sample:
20nt DNA