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bioZen SPE solation and Cleanup of Biomolecules: Phenomenex
bioZen sample preparation products allow for isolation and cleanup of proteins and peptides. bioZen sample preparation products include solid phase extraction plates for specialty applications such as recovery of N-Glycan and bioZen magnetic beads for targeted isolation of proteins and peptides
bioZen Sample Preparation Products
Explore a New Sample Preparation State of Zen

MagBeads

Streptavidin coated paramagnetic particle, ideal for isolating proteins and peptides in less time with a high response. With a unique magnetic bead particle and rigorous specifications, bioZen MagBeads are created to ensure uniform beads that provide consistent and reliable results. Pairs with: bioZen XB-Peptide and Peptide LC Columns

 

N-Glycan Clean-Up SPE

Solid phase extraction (SPE) HILIC stationary phase that excels at retention and recovery labeled released N-glycans! Microelution format allows for streamlined processing and clean-up of small sample volumes. Pair with: bioZen Glycan LC Columns

Peptide MagBeads

Streptavidin Coated

Uniform magnetic particles result in faster and reliable purification, clean-up, and isolation of proteinsand peptide molecules.

MagaBead Activation

Immunocapture

Glycan: N-Glycan Clean-Up

Streptavidin Coated

Uniform magnetic particles result in faster and reliable purification, clean-up, and isolation of proteins and peptide molecules.

STEP 1

STEP 2

STEP 3

STEP 4

STEP 5
Start with Intact Glycoprotein Deglycosylation
Released N-Glycans by PNGase F in buffer solution
Labeling Glycans
2-AA or 2-AB for accurate and sensitive glycan detection 
Clean-Up
Removal of labeling reagent, salts, or surfactants
Analysis

96-Well Plate:
Part No.:
8M-S009-NGA
Condition:
200 µL Water
Equilibrate:
200 µL Water/Acetonitrile (15:85)
Load:
Labeled Sample (up to 400 µL of labeled sample)
Wash:
2x 600 µL Formic acid/Water/Acetonitrile (1:9:90)
Elute:
3x 30 µL 200 mM Ammonium acetate in Acetonitrile/Water (5:95) of labeled sample)
Dilute:
100 µL Dimethylformamide and then 210 µL of Acetonitrile

Pair with a bioZen LC Column
Intact
bioZen Intact XB-C8
3.6 µm
bioZen WidePore C4
2.6 µm

Large pore core-shell particle for fast intact and subunit biologic entry. C8 provides highly useful moderate hydrophobic selectivity.


Core-shell particle with butyl stationary phase and optimal wide pore size distribution for better resolution of large biologics, including monoclonal antibodies and subunit analysis.



Size Exclusion (SEC)
bioZen SEC-2
1.8 µm
bioZen SEC-3
1.8 µm

Large pore core-shell particle for fast intact and subunit biologic entry. C8 provides highly useful moderate hydrophobic selectivity.


Extremely inert, high density fully porous particle with high efficiency and high molecular weight (HMW) separation range of 10 k – 700 kDa.


Peptide
bioZen Peptide XB-C18
1.7 µm and 2.6 µm
bioZen Peptide PS-C18
1.6 µm and 3 µm

Overall retention of both acidic and basic peptides through C18 stationary phase with di-isobutyl side chains.


Excellent retention by combined positively charged surface ligand and C18 ligand.


Ion-Exchange Glycan
bioZen WCX
6 µm
bioZen Glycan
2.6 µm

Monosized particles grafted with linear polycarboxylate chains to envelop and separate proteins from acidic/basic variants

Provides optimal combination of high efficiency and selectivity for released glycans.


Oligonucleotides
bioZen Oligo
1.7 µm and 2.6 µm

Organo-silica core-shell particle bonded with a C18 stationary phase offers high selectivity for even minute oligo differences alongside high and low pH robustness.