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Axia Pre-packed Preparative HPLC and SFC Columns: Phenomenex
Axia pre-packed preparative HPLC columns are available in 20.2, 30.0, and 50 mm IDs and offers improved lifetime and performance over traditionally packed preparative columns
Axia Preparative Technology
Guaranteed, Longest Lasting Preparative HPLC and SFC Column

Available in over 40 unique achiral and chiral selectivities, Axia advanced preparative column packings and column hardware designs offer several advantages. Unlike traditional preparative LC column packing methods, the Axia packing method offers increased sorbent bed density for increased performance and eliminates media bed collapse as a source of premature column failure in preparative HPLC/SFC columns.


The Ultimate Pre-Packed Preparative Coumn for HPLC and SFC

Axia Preparative LC Columns
Axia Packed Preparative Columns

Axia Single Axial Compression Step

Axia packed preparative columns involve a single axial compression step, unlike conventional packed preparative columns. The ideal column bed density is custom calculated and automated for each specific media and column size. Computer control of the entire process ensures both proper bed density and column uniformity every time.

Axia packed columns produce uniform media bed with intact particles
*SEM of Axia inlet frit
Axia™ packed columns produce uniform media bed with intact particles

The highly tuned patented process and hardware eliminates potential decompression ensuring bed stability and optimal packing density. The media found on the inlet frit of the Axia packed column shows no signs of damage unlike the media found on inlet frit of traditionally packed prep columns. 

Traditional packed preparative columns produce non-uniform media beds with sheared and crushed particles
*SEM of Waters® OBD™ inlet frit
Traditional packed preparative columns produce non-uniform media beds with sheared and crushed particles

Decompression and then recompression during packing can damage the media and lead to increased column-to-column variability, flow disturbances, and decreased column lifetimes.

Unmatched Column Reproducibility

The automated Axia ™ packing system provides feedback control and infinite tuning of packing density for specific media characteristics such as mechanical strength and porosity. An optimum higher bed density can be consistently reproduced column-to-column. This directly translates into consistent efficiency and peak asymmetry measurements and decreases the column variability seen in traditionally packed preparative columns.

Unmatched Column Reproducibility with  Axia Preparative LC columns



Compare Lifetime

Comparison of Axia Lifetime with Traditional Preparative LC
Comparative separations may not be representative of all applications. ‡Same linear velocity
Conditions for all columns:

Columns:
Waters® XTerra® 5 µm Prep MS C18 OBD™
Dimension:
50 x 21.2 mm (Luna)
50 x 19 mm (XTerra)
Mobile Phase:
A: 0.5 % TFA in Water
B: 0.5 % TFA in Acetonitrile Formic Acid
Gradient:
Linear 95:5 (A/B) to 5:95 (A/B)
over 7 min, hold 3 min
Flow Rate:
30 mL/min (Luna)
24 mL/min (XTerra)
Temperature:
Ambient
Injection Volume:
500 μL
Detection:
UV @ 254 nm
Sample:
1. Triprolidine 1.6 mg
2. Methacycline 16 mg
3. Amitriptyline 5.25 mg

Seamless Scalability to Increase Sample Load

Increase Column Length

Increase sample load without increasing your flow rate by using a longer column. With Axia technology, each preparative column is optimized for:
  • Analytical-like efficiency
  • Long column lifetime
  • High sample load with high surface area media such as Kinetex, Aeris, Gemini, Luna, Luna Omega, or Synergi

Loadability Increases with Column Performance on Axia Preparative LC Columns
Condition:

Gradient:
10 min on 100 mm length column 25 min on 250 mm length column 5 % to 95 % Acetonitrile, 0.5 % TFA
Flow Rate:
30 mL/min

Increase Column ID

For maximizing load without increasing the run time, consider scaling up to a larger column ID. Axia packed columns provide the three important benefits you need.
  • Reproducible performance across all column diameters
  • Increased throughput without sacrificing purity
  • High efficiency from analytical to preparative

Analytical 50 x 4.6 mm

Standard loading on a Analytical 50 x 4.6 mm

Dimension:
50 x 4.6 mm
Sample Load:
0.1 mg in 21 µL DMSO
Flow Rate
1.5 mL/min

Axia PREP 50 x 21.2 mm

Loading on a Axia Preparative LC Column 50 x 21.2 mm

Dimension:
50 x 21.2 mm
Sample Load:
2.1 mg in 420 µL DMSO
Flow Rate
1.5 mL/min

Axia PREP 50 x 30 mm

Axia Preparative LC Column 50 x 30 mm

Dimension:
50 x 30 mm
Sample Load:
4.2 mg in 840 µL DMSO
Flow Rate
60 mL/min

Axia PREP 50 x 50 mm

Axia Preparative LC Column 50 x 50 mm

Dimension:
50 x 50 mm
Sample Load:
10.5 mg in 2100 µL DMSO
Flow Rate
150 mL/min

Axia Technology Outperforms Traditional Packing Processes!
Because of the constant pressure placed on the integrated packing piston, Axia packed columns possess the dynamic capability of maintaining a consistent, homogeneous media bed. This results in superior column performance no matter which media selectivity you choose.

Warfarin Chiral Purification in Normal Phase Mode

Warfarin Chiral Purification in Normal Phase Mode using a Lux Cellulose-1 Analytical Column

Column:
Dimension:
150 x 4.6 mm
Mobile Phase:
Hexane / Ethanol (75:25)
Flow Rate:
1 mL/min
Temperature:
Ambient
Injection Volume:
100 µL

Warfarin Chiral Purification in Normal Phase Mode on  Axia Prep LC Column
Conditions for both PREP columns:

Column:
Dimension:
150 x 21.1 mm
Mobile Phase:
Hexane / Ethanol (75:25)
Flow Rate:
20 mL/min
Temperature:
Ambient
Injection Volume:
20 mL

42 % Increase in Axia Efficiency

Column (mm) Analytical
150 x 4.6
Standard
150 x 21.2
Axia
150 x 21.2
Mass Loaded (mg) 2 40 40
Resolution* 1.5 2.85 3.72
Plates (N) 117 535 760

Axia column and packing technology is patented by Phenomenex. U.S. Patent No. 7, 674, 383