Applications
Dexamethasone USP Standard Solution on Kinetex 1.7 µm C18 100 x 2.1 mm on Agilent 1290 UHPLC
26286
Separation Mode: Reversed Phase
Reversed Phase
USP
C18
HPLC
Antineoplastic Agents
Pharmaceutical/Biopharmaceutical
Pharmaceutical
Therapeutic / Clinical

Applications

Dexamethasone USP Standard Solution on Kinetex 1.7 µm C18 100 x 2.1 mm on Agilent 1290 UHPLC

Analytes

1
Compound Name
Dexamethasone
CID
5743
Molecular Formula
C22H29FO5
Molecular Weight
392
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
3

Conditions

LC Conditions (App ID: 26286)
Official Method:USP
Column:Kinetex 1.7 µm C18 100 Å, LC Column 100 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00D-4475-AN
Elution Type:Gradient
Mobile Phase:A: 3.4 g/L of monobasic potassium phosphate in water. Adjust with phosphoric acid to a pH of 3.0
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
107624
2107624
3154555
4161090
516.17624
6207624
Flow Rate:0.4 mL/min
Temperature:35°C
System:Agilent Technologies Agilent Technologies
Detection:LC/UV (DAD, PDA) nm (nanometers) @ 0°C

Order Items Used in This Application

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