Applications
USP Sildenafil Citrate Tablet Assay - Sensitivity Solution 0.00035 mg/mL on Kinetex 5 µm C18 150x4.6mm LC/UV
26208
Separation Mode: Reversed Phase
Reversed Phase
USP
C18
HPLC
Pharmaceutical/Biopharmaceutical
Pharmaceutical
Therapeutic / Clinical

Applications

USP Sildenafil Citrate Tablet Assay - Sensitivity Solution 0.00035 mg/mL on Kinetex 5 µm C18 150x4.6mm LC/UV
Analytes
1
Compound Name
Sildenafil
CID
5212
Molecular Formula
C22H30N6O4S
Molecular Weight
474
No. Hydrogen Bond Acceptors
8
No. Hydrogen Bond Donors
1
Details
Official Method: USP
LC Conditions (App ID: 26208)
Column:
Brand Name:
Part No:
Elution Type:
Isocratic
Mobile Phase:
A: Buffer (dilute 7 mL of triethylamine with water to 1 L. Stir and adjust with phosphoric acid to a pH of 3.0+/- 0.1), Methanol and Acetonitrile (58:25:17)
Gradient Profile:
Step No.Time(min)%A
120100
Flow Rate:
1.4 mL/min
Temperature:
30°C
System:
Agilent Technologies 1100 Series Agilent Technologies 1100 Series
Detection:
LC/UV (DAD, PDA) nm (nanometers) @ 30°C

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