Applications
Clean-Up of Phospholipids in Human Plasma Comparing PPT VS. Strata-X PRO SPE on a Kinetex 2.6 µm C18 50 x 2.1 mm
25779
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Clinical
Clinical Research
Therapeutic / Clinical

Applications

Clean-Up of Phospholipids in Human Plasma Comparing PPT VS. Strata-X PRO SPE on a Kinetex 2.6 µm C18 50 x 2.1 mm

Analytes

1
2
3

Analyte not found

Conditions

LC Conditions (App ID: 25779)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00B-4462-AN
Elution Type:Gradient
Mobile Phase:A: 0.1% Formic acid in water
B: 0.1% Formic acid in methanol
Gradient Profile:
Step No.Time(min)%A%B
106040
20.5595
311.5595
411.516040
513.56040
Flow Rate:400 μL/min
Temperature:40°C
System:Agilent Technologies Agilent Technologies
Detection:LC/MS/MS @ 40°C

Sample Preparation Details
Description:Strata-X PRO, 96-Well Plate, 30mg/well, Ea
Part Number:8E-S536-TGA
Pre Treatment Note:Sample Pre-treatment: • Add 100 µL Serum to tube • Add 900 µL of chilled (~0°C) ACN to the serum while vortexing • Centrifuge samples at 6000 rpm for 5 minutes and transfer supernatant

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