Applications
Extraction of 5-Fluorouracil from Human Serum Using Strata-X PRO SPE on a Kinetex 2.6 μm PS C18 (ESI -Ve Mode)
25778
Separation Mode: Reversed Phase
Reversed Phase
PS C18
HPLC
Clinical
Clinical Research
Therapeutic / Clinical

Applications

Extraction of 5-Fluorouracil from Human Serum Using Strata-X PRO SPE on a Kinetex 2.6 μm PS C18 (ESI -Ve Mode)

Analytes

1
2
Compound Name
5-Fluorouracil
CID
3385
Molecular Formula
C4H3FN2O2
Molecular Weight
130
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
2

Conditions

LC Conditions (App ID: 25778)
Column:Kinetex 2.6 µm PS C18 100 Å, LC Column 150 x 3 mm, Ea
Brand Name:Kinetex
Part No:00F-4780-Y0
Elution Type:Gradient
Mobile Phase:A: 0.1% Formic acid in Water
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
10937
212937
Flow Rate:0.2 mL/min
Temperature:25°C
System:Agilent Technologies 1260 Infinity Agilent Technologies 1260 Infinity
Detection:LC/MS/MS m/z (mass per charge) @ 25°C

Sample Preparation Details
Description:Strata-X PRO, 96-Well Plate, 30mg/well, Ea
Part Number:8E-S536-TGA
Pre Treatment Note:Sample Pre-treatment: • Add 100 µL Serum to tube • Add 900 µL of chilled (~0°C) ACN to the serum while vortexing • Centrifuge samples at 6000 rpm for 5 minutes and transfer supernatant

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