Applications
Extraction of 5-Fluorouracil from Human Serum Using Strata-X PRO SPE on a Kinetex 2.6 μm PS C18 (ESI -Ve Mode)
25778
Separation Mode: Reversed Phase
Reversed Phase
PS C18
HPLC
Clinical
Clinical Research
Therapeutic / Clinical

Applications

Extraction of 5-Fluorouracil from Human Serum Using Strata-X PRO SPE on a Kinetex 2.6 μm PS C18 (ESI -Ve Mode)
Analytes
1
2
Compound Name
5-Fluorouracil
Details
LC Conditions (App ID: 25778)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% Formic acid in Water
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
10937
212937
Flow Rate:
0.2 mL/min
Temperature:
25°C
System:
Agilent Technologies 1260 Infinity Agilent Technologies 1260 Infinity
Detection:
LC/MS/MS m/z (mass per charge) @ 25°C
Sample Preparation Details
Description:
Strata-X PRO, 96-Well Plate, 30mg/well, Ea
Part Number:
Pre Treatment Note:
Sample Pre-treatment: • Add 100 µL Serum to tube • Add 900 µL of chilled (~0°C) ACN to the serum while vortexing • Centrifuge samples at 6000 rpm for 5 minutes and transfer supernatant

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