Extraction of Uracil and Dihydrouracil from Human Serum Using Strata-X PRO SPE Using Kinetex 2.6 μm PS C18 (ESI +Ve mode)

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Applications

25777

Separation Mode: Reversed Phase

Reversed Phase

PS C18

HPLC

Clinical

Clinical Research

Therapeutic / Clinical

App ID 25777

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Extraction of Uracil and Dihydrouracil from Human Serum Using Strata-X PRO SPE Using Kinetex 2.6 μm PS C18 (ESI +Ve mode)

Order Items Used in This Application

00F-4780-Y0

Kinetex 2.6 µm PS C18 100 Å, LC Column 150 x 3 mm, Ea

Details

LC Conditions

Column:

Kinetex 2.6 µm PS C18 100 Å, LC Column 150 x 3 mm, Ea

Brand Name:

Kinetex

Part No:

00F-4780-Y0

Elution Type:

Gradient

Mobile Phase:

A: 0.1%Formic acid in Water
B: Methanol

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	93	7
2	12	93	7

Flow Rate:

0.2 mL/min

Temperature:

25°C

System:

Agilent Technologies 1260 Infinity Agilent Technologies 1260 Infinity

Detection:

[object Object]

LC Sample Preparation Details

Description:	Strata-X PRO, 96-Well Plate, 30mg/well, Ea
Part Number:	8E-S536-TGA
Pre Treatment Note:	Sample Pre-treatment: • Add 100 µL Serum to tube • Add 900 µL of chilled (~0°C) ACN to the serum while vortexing • Centrifuge samples at 6000 rpm for 5 minutes and transfer supernatant