Applications

Extraction of Uracil and Dihydrouracil from Human Serum Using Strata-X PRO SPE Using Kinetex 2.6 μm PS C18 (ESI +Ve mode)

Analytes

Analyte not found

Details

LC Conditions (App ID: 25777)

Elution Type:

Gradient

Mobile Phase:

A: 0.1%Formic acid in Water

B: Methanol

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	93	7
2	12	93	7

Flow Rate:

0.2 mL/min

Temperature:

25°C

System:

Agilent Technologies 1260 Infinity Agilent Technologies 1260 Infinity

Detection:

LC/MS/MS m/z (mass per charge) @ 25°C

Sample Preparation Details

Pre Treatment Note:

Sample Pre-treatment: • Add 100 µL Serum to tube • Add 900 µL of chilled (~0°C) ACN to the serum while vortexing • Centrifuge samples at 6000 rpm for 5 minutes and transfer supernatant

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Applications

Extraction of Uracil and Dihydrouracil from Human Serum Using Strata-X PRO SPE Using Kinetex 2.6 μm PS C18 (ESI +Ve mode)

LC Application

App ID 25777

Applications

Extraction of Uracil and Dihydrouracil from Human Serum Using Strata-X PRO SPE Using Kinetex 2.6 μm PS C18 (ESI +Ve mode)shareLinkedinprintfavorite_border

LC Application

App ID 25777

Extraction of Uracil and Dihydrouracil from Human Serum Using Strata-X PRO SPE Using Kinetex 2.6 μm PS C18 (ESI +Ve mode)