Applications
Berberine extracted from Goldenseal pill on Kinetex PS-C18
25524
Separation Mode: Reversed Phase
Reversed Phase
PS C18
HPLC
Food & Beverage
Food and Beverage (incl. additives
contaminants)
Product / Trade Good

Applications

Berberine extracted from Goldenseal pill on Kinetex PS-C18

Analytes

1
Compound Name
Berberine
CID
2353
Molecular Formula
C20H18NO4+
Molecular Weight
336
No. Hydrogen Bond Acceptors
4
Total Formal Charge
1

Conditions

LC Conditions (App ID: 25524)
Column:Kinetex 2.6 µm PS C18 100 Å, LC Column 50 x 4.6 mm, Ea
Brand Name:Kinetex
Part No:00B-4780-E0
Guard Cartridge:AJ0-8949
Elution Type:Gradient
Mobile Phase:A: 20mM Potassium dihydrogen phosphate pH 4.6
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
109010
273565
383565
48.59010
5109010
Flow Rate:1.5 mL/min
Temperature:23°C
System:Agilent Technologies 1100 Series Agilent Technologies 1100 Series
Detection:LC/UV (DAD, PDA) nm (nanometers) @ 0°C

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