Applications
Removal of Phospholipids from Milk Sample Using Strata-X PRO With a Kinetex 2.6 µm C18 50 x 2.1 mm
25488
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Food & Beverage
Environmental / Health
Product / Trade Good

Applications

Removal of Phospholipids from Milk Sample Using Strata-X PRO With a Kinetex 2.6 µm C18 50 x 2.1 mm
Analytes
1
2
3

Analyte not found

Details
LC Conditions (App ID: 25488)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1%Formic acid in Water
B: 0.1%Formic acid in Methanol
Gradient Profile:
Step No.Time(min)%A%B
106040
20.5595
311.5595
411.516040
5136040
Flow Rate:
0.4 mL/min
Temperature:
45°C
System:
Agilent Technologies 1260 Infinity Agilent Technologies 1260 Infinity
Detection:
LC/MS/MS nm (nanometers) @ 30°C
Sample Preparation Details
Description:
Strata-X PRO, 60mg/3mL, 50/Pk
Part Number:
Pre Treatment Note:
To 1 mL of milk add 3 mL of 0.2% formic acid in Acetonitrile /Methanol (90:10). Mix/vortex for 15-20 secs. Centrifuge for 5 min at 10,000 rpm. Collect supernatant to perform SPE in next step.

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