Applications
Removal of Phospholipids from Milk Sample Using Strata-X PRO With a Kinetex 2.6 µm C18 50 x 2.1 mm
25488
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Food & Beverage
Environmental / Health
Product / Trade Good

Applications

Removal of Phospholipids from Milk Sample Using Strata-X PRO With a Kinetex 2.6 µm C18 50 x 2.1 mm

Analytes

1
2
3

Analyte not found

Conditions

LC Conditions (App ID: 25488)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00B-4462-AN
Elution Type:Gradient
Mobile Phase:A: 0.1%Formic acid in Water
B: 0.1%Formic acid in Methanol
Gradient Profile:
Step No.Time(min)%A%B
106040
20.5595
311.5595
411.516040
5136040
Flow Rate:0.4 mL/min
Temperature:45°C
System:Agilent Technologies 1260 Infinity Agilent Technologies 1260 Infinity
Detection:LC/MS/MS nm (nanometers) @ 30°C

Sample Preparation Details
Description:Strata-X PRO, 60mg/3mL, 50/Pk
Part Number:8B-S536-UBJ
Pre Treatment Note:To 1 mL of milk add 3 mL of 0.2% formic acid in Acetonitrile /Methanol (90:10). Mix/vortex for 15-20 secs. Centrifuge for 5 min at 10,000 rpm. Collect supernatant to perform SPE in next step.

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