Applications
Extraction of Vet Drugs in Milk Using Strata-X PRO with a Kinetex 2.6 µm Biphenyl 50 x 3.0mm
25487
Separation Mode: Reversed Phase
Reversed Phase
Biphenyl
HPLC
Antineoplastic Agents
Food & Beverage
Environmental / Health
Product / Trade Good

Applications

Extraction of Vet Drugs in Milk Using Strata-X PRO with a Kinetex 2.6 µm Biphenyl 50 x 3.0mm
Analytes
1
2
3
4
5
6
7
8
9
10
11
12
13
Compound Name
Sulfaguanidine
Details
LC Conditions (App ID: 25487)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1%Formic acid in Water
B: 0.1%Formic acid in Methanol
Gradient Profile:
Step No.Time(min)%A%B
10955
21.5595
33595
43.01955
54.5955
Flow Rate:
0.5 mL/min
Temperature:
45°C
System:
Agilent Technologies 1260 Infinity Agilent Technologies 1260 Infinity
Detection:
LC/MS/MS nm (nanometers) @ 30°C
Sample Preparation Details
Description:
Strata-X PRO, 60mg/3mL, 50/Pk
Part Number:
Pre Treatment Note:
To 1 mL of milk add 3 mL of 0.2% formic acid in Acetonitrile /Methanol (90:10). Mix/vortex for 15-20 secs. Centrifuge for 5 min at 10,000 rpm. Collect supernatant to perform SPE in next step.

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Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 2.1 mm, Ea
Reversed Phase