Applications
Water Soluble Vitamins on Kinetex 2.6u Polar 150x4.6mm
24270
Vitamin B Complex

Applications

Water Soluble Vitamins on Kinetex 2.6u Polar 150x4.6mm
Analytes
1
2
3
4
5
Compound Name
Thiamin
CID
1130
Molecular Formula
C12H17N4OS+
Molecular Weight
265
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
2
Total Formal Charge
1
Details
LC Conditions (App ID: 24270)
Elution Type:
Gradient
Mobile Phase:
A: 20mM Potassium Phosphate pH 3.5
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
101000
2107030
3117030
411.51000
5141000
Flow Rate:
1.2 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C
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