Applications
Chromatographic Separation of Isomeric UDCA, CDCA and DCA on Kinetex 2.6µm Polar C18 100x2.1 Column
24193
Separation Mode: Reversed Phase
Reversed Phase
Polar C18
HPLC
Gastrointestinal Agents
Clinical
Clinical Research
Therapeutic / Clinical

Applications

Chromatographic Separation of Isomeric UDCA, CDCA and DCA on Kinetex 2.6µm Polar C18 100x2.1 Column

Analytes

1
2
3
Compound Name
Actigall
CID
465690
Molecular Formula
C24H40O4
Molecular Weight
392
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
3

Conditions

LC Conditions (App ID: 24193)
Column:Kinetex 2.6 µm Polar C18 100 Å, LC Column 100 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00D-4759-AN
Elution Type:Gradient
Mobile Phase:A: 2mM Ammonium acetate (pH 6.9)
B: Methanol/Acetonitrile (50-50)
Gradient Profile:
Step No.Time(min)%A%B
105545
293070
39.53070
49.515545
5125545
Flow Rate:400 mL/min
Temperature:50°C
Detection:Mass Spectrometer (MS) amu @ 50°C

Order Items Used in This Application

Similar Applications
Kinetex 2.6 µm Polar C18 100 Å, LC Column 100 x 2.1 mm, Ea
Reversed Phase