Applications
uCATs by LCMS using Kinetex 2.6um Polar C18 50x4.6mm
24172
Clinical
Clinical Research
Therapeutic / Clinical

Applications

uCATs by LCMS using Kinetex 2.6um Polar C18 50x4.6mm
Analytes
1
2
3
Compound Name
Metanephrine
CID
21100
Molecular Formula
C10H15NO3
Molecular Weight
197
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 24172)
Elution Type:
Gradient
Mobile Phase:
A: Water with 0.1% formic acid
B: Methanol with 0.1% formic acid
Gradient Profile:
Step No.Time(min)%A%B
101000
230100
Flow Rate:
0.7 mL/min
Temperature:
25°C
Detection:
Tandem Mass Spec (MS-MS) @ 22°C
Sample Preparation Details
Pre Treatment Note:
Pool eluents and dilute with 100 µL of 0.1% Formic acid in water
SPE Condition 1:
0.2 mL Methanol
SPE Condition 2:
0.2 mL 50 mM Ammonium acetate, pH 7
SPE Load:
Load 1.00 mL Sample diluted in Pretreated sample
SPE Wash 1:
0.2 mL 50 mM Ammonium acetate, pH 7
SPE Wash 2:
0.2 mL Acetonitrile/ Isopropyl alcohol (1:1)
SPE Dry:
3 minutes under high vacuum
SPE Elution 1:
0.025 mL Water / Acetonitrile / Formic acid (85:10:5)
SPE Elution 2:
0.025 mL Water / Acetonitrile / Formic acid (85:10:5)
SPE Evaporation:
Evaporate to dryness