Applications
uCATs by LCMS using Kinetex 2.6um Polar C18 50x4.6mm
24172
Separation Mode: Reversed Phase
Reversed Phase
Polar C18
HPLC
Clinical
Clinical Research
Therapeutic / Clinical

Applications

uCATs by LCMS using Kinetex 2.6um Polar C18 50x4.6mm
Analytes
1
2
3
Compound Name
Metanephrine
Details
LC Conditions (App ID: 24172)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: Water with 0.1% formic acid
B: Methanol with 0.1% formic acid
Gradient Profile:
Step No.Time(min)%A%B
101000
230100
Flow Rate:
0.7 mL/min
Temperature:
25°C
Detection:
Tandem Mass Spec (MS-MS) @ 22°C
Sample Preparation Details
Description:
Strata-X-CW Polymeric Weak Cation, microelution 96-well plate, 2mg/well, Ea
Part Number:
Pre Treatment Note:
Pool eluents and dilute with 100 µL of 0.1% Formic acid in water
SPE Condition 1:
0.2 mL Methanol
SPE Condition 2:
0.2 mL 50 mM Ammonium acetate, pH 7
SPE Load:
Load 1.00 mL Sample diluted in Pretreated sample
SPE Wash 1:
0.2 mL 50 mM Ammonium acetate, pH 7
SPE Wash 2:
0.2 mL Acetonitrile/ Isopropyl alcohol (1:1)
SPE Dry:
3 minutes under high vacuum
SPE Elution 1:
0.025 mL Water / Acetonitrile / Formic acid (85:10:5)
SPE Elution 2:
0.025 mL Water / Acetonitrile / Formic acid (85:10:5)
SPE Evaporation:
Evaporate to dryness

Order Items Used in This Application

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