Applications
Water Soluble Vitamins on Kinetex 2.6u Polar C18 100x4.6mm
24038
Separation Mode: Reversed Phase
Reversed Phase
Polar C18
HPLC
Vitamin B Complex
Food & Beverage
Food and Beverage (incl. additives
contaminants)
Product / Trade Good

Applications

Water Soluble Vitamins on Kinetex 2.6u Polar C18 100x4.6mm

Analytes

1
2
3
4
5
6
Compound Name
Thiamin
CID
1130
Molecular Formula
C12H17N4OS+
Molecular Weight
265
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
2
Total Formal Charge
1

Conditions

LC Conditions (App ID: 24038)
Column:Kinetex 2.6 µm Polar C18 100 Å, LC Column 100 x 4.6 mm, Ea
Brand Name:Kinetex
Part No:00D-4759-E0
Elution Type:Gradient
Mobile Phase:A: 20mM Potassium Phosphate pH 3.5
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
101000
211000
3104060
4114060
511.11000
6151000
Flow Rate:1.2 mL/min
Temperature:22°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

Order Items Used in This Application

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