Applications
Bupropion Extracted from Urine using Strata X-CW and Kinetex 5u EVO C18
23095
Separation Mode: Reversed Phase
Reversed Phase
EVO C18
HPLC
Antidepressive Agents, Second-Generation
Pharmaceutical/Biopharmaceutical
Therapeutic / Clinical
Therapeutic / Clinical

Applications

Bupropion Extracted from Urine using Strata X-CW and Kinetex 5u EVO C18
Analytes
1
Compound Name
Bupropion
Details
LC Conditions (App ID: 23095)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 5mM Ammonium Bicarbonate pH 9.0
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
105050
2102080
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C
Sample Preparation Details
Description:
Strata-X-CW 33 µm Polymeric Weak Cation, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:
SPE Condition 1:
3 mL Metahnol at 1 mL/min
SPE Condition 2:
3 mL Water at 1 mL/min
SPE Load:
Load 1.00 mL Sample diluted in 100mM Sodium Phosphate pH 6.8
SPE Wash 1:
3 mL 100mM Sodium Phosphate pH 6.8 at 1 mL/min
SPE Dry:
5 minutes
SPE Elution 1:
2 mL 5% Formic Acid in Acetonitrile at 0.5 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
500 µL 50:50 5mM Ammonium Bicarbonate pH 9.0 and Methanol

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