Applications
Amino Acid Analysis using OPA on Kinetex 5u EVO C18 150x4.6mm
23092
Separation Mode: Reversed Phase
Reversed Phase
EVO C18
HPLC
Glycine Agents
Pharmaceutical/Biopharmaceutical
Life Science
Therapeutic / Clinical
Biochemical Compound / Nutrient
Therapeutic / Clinical

Applications

Amino Acid Analysis using OPA on Kinetex 5u EVO C18 150x4.6mm

Analytes

1
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Compound Name
L-Aspartic Acid
CID
5960
Molecular Formula
C4H7NO4
Molecular Weight
133
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
3

Conditions

LC Conditions (App ID: 23092)
Column:Kinetex 5 µm EVO C18 100 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Kinetex
Part No:00F-4633-E0
Elution Type:Gradient
Mobile Phase:A: 20mM Potassium Phosphate pH 7.2
B: 50:50 Acetonitrile:Methanol
Gradient Profile:
Step No.Time(min)%A%B
10973
2204060
323973
Flow Rate:1 mL/min
Temperature:30°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

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