Applications
Nicotine & Metabolites on Kinetex 2.6µm Evo C18 100x3mm
23006
Separation Mode: Reversed Phase
Reversed Phase
EVO C18
HPLC
Nicotinic Agonists
Pharmaceutical/Biopharmaceutical
Clinical
Forensics/Toxicology
Therapeutic / Clinical
Therapeutic / Clinical

Applications

Nicotine & Metabolites on Kinetex 2.6µm Evo C18 100x3mm

Analytes

1
2
3
4
5
Compound Name
Hydroxycotinine
CID
414
Molecular Formula
C10H12N2O2
Molecular Weight
192
No. Chiral Centers:
2
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 23006)
Column:Kinetex 2.6 µm EVO C18 100 Å, LC Column 100 x 3.0 mm, Ea
Brand Name:Kinetex
Part No:00D-4725-Y0
Elution Type:Gradient
Mobile Phase:A: 20mM Ammonium Bicarbonate, pH 8.2
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
109010
231090
351090
45.019010
569010
Flow Rate:750 µL/min
Temperature:25°C
Detection:Mass Spectrometer (MS) amu @ 0°C

Order Items Used in This Application

Similar Applications
Kinetex 5 µm EVO C18 100 Å, LC Column 100 x 3.0 mm, Ea
Reversed Phase