Applications
Extraction of Timolol from Serum using Strata-X-C 10 mg Plate & Kinetex 2.6u Biphenyl 50x2.1 Column
22982
Separation Mode: Reversed Phase
Reversed Phase
Biphenyl
HPLC
Antihypertensive Agents

Applications

Extraction of Timolol from Serum using Strata-X-C 10 mg Plate & Kinetex 2.6u Biphenyl 50x2.1 Column
Analytes
1
Compound Name
Timolol
Details
LC Conditions (App ID: 22982)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% formic acid in water
B: 0.1% formic acid in acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10955
20.5955
33595
43.5595
53.51955
65.5955
Flow Rate:
0.5 mL/min
Temperature:
25°C
Detection:
Tandem Mass Spec (MS-MS) @ 22°C
Sample Preparation Details
Description:
Strata X-C 33 µm Polymeric Strong Cation, 10 mg / well, 96-Well Plates, 2/Pk
Part Number:
Pre Treatment Note:
No dry down step was performed. 1µL of final eluent was injected.
SPE Condition 1:
0.5 mL Methanol
SPE Condition 2:
0.5 mL Water
SPE Load:
Load 375.00 µL Sample diluted in 375 µL 4% Phosphoric acid in water
SPE Wash 1:
0.5 mL 2% Formic acid in water
SPE Wash 2:
0.5 mL Methanol
SPE Elution 1:
0.125 mL 5% Ammonium hydroxide in Acetonitrile/Methanol (60:40)
SPE Elution 2:
0.125 mL 5% Ammonium hydroxide in Acetonitrile/Methanol (60:40)
SPE Elution 3:
0.125 mL 5% Ammonium hydroxide in Acetonitrile/Methanol (60:40)
SPE Evaporation:
Evaporate to dryness

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