Applications
Extraction of Timolol from Serum using Strata-X-C 10 mg Plate & Kinetex 2.6u Biphenyl 50x2.1 Column
22982
Separation Mode: Reversed Phase
Reversed Phase
Biphenyl
HPLC
Antihypertensive Agents

Applications

Extraction of Timolol from Serum using Strata-X-C 10 mg Plate & Kinetex 2.6u Biphenyl 50x2.1 Column

Analytes

1
Compound Name
Timolol
CID
33624
Molecular Formula
C13H24N4O3S
Molecular Weight
316
No. Hydrogen Bond Acceptors
7
No. Hydrogen Bond Donors
2

Conditions

LC Conditions (App ID: 22982)
Column:Kinetex 2.6 µm Biphenyl 100 Å, LC Column 50 x 2.1 mm, Ea
Brand Name:Kinetex
Part No:00B-4622-AN
Elution Type:Gradient
Mobile Phase:A: 0.1% formic acid in water
B: 0.1% formic acid in acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10955
20.5955
33595
43.5595
53.51955
65.5955
Flow Rate:0.5 mL/min
Temperature:25°C
Detection:Tandem Mass Spec (MS-MS) @ 22°C

Sample Preparation Details
Description:Strata X-C 33 µm Polymeric Strong Cation, 10 mg / well, 96-Well Plates, 2/Pk
Part Number:8E-S029-AGB
Pre Treatment Note:No dry down step was performed. 1µL of final eluent was injected.
SPE Condition 1:0.5 mL Methanol
SPE Condition 2:0.5 mL Water
SPE Load:Load 375.00 µL Sample diluted in 375 µL 4% Phosphoric acid in water
SPE Wash 1:0.5 mL 2% Formic acid in water
SPE Wash 2:0.5 mL Methanol
SPE Elution 1:0.125 mL 5% Ammonium hydroxide in Acetonitrile/Methanol (60:40)
SPE Elution 2:0.125 mL 5% Ammonium hydroxide in Acetonitrile/Methanol (60:40)
SPE Elution 3:0.125 mL 5% Ammonium hydroxide in Acetonitrile/Methanol (60:40)
SPE Evaporation:Evaporate to dryness

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