Applications
Steroids mix using Kinetex 5u C18 150x4.6mm
22952
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Anti-Inflammatory Agents

Applications

Steroids mix using Kinetex 5u C18 150x4.6mm

Analytes

1
2
3
4
5
Compound Name
Hydrocortisone
CID
5754
Molecular Formula
C21H30O5
Molecular Weight
362
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
3

Conditions

LC Conditions (App ID: 22952)
Column:Kinetex 5 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Kinetex
Part No:00F-4601-E0
Elution Type:Isocratic
Mobile Phase:A: 0.1% H3PO4 in Water
B: Acetontrile
C: Methanol
Gradient Profile:
Step No.Time(min)%A%B%C
10543511
Flow Rate:1 mL/min
Temperature:22°C
Detection:UV-Vis Abs.-Diode Array (PDA) nm @ 22°C

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