Applications
Ascorbic Acid by HPLC Standard
22905
Separation Mode: Reversed Phase
Reversed Phase
Hydro-RP
HPLC
Antioxidants

Applications

Ascorbic Acid by HPLC Standard

Analytes

1
Compound Name
Ascorbic Acid
CID
5785
Molecular Formula
C6H8O6
Molecular Weight
176
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
4

Conditions

LC Conditions (App ID: 22905)
Column:Synergi 4 µm Hydro-RP 80 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Synergi
Part No:00F-4375-E0
Elution Type:Isocratic
Mobile Phase:A: 0.1% H3PO4 in Water
Gradient Profile:
Step No.Time(min)%A
10100
Flow Rate:1 mL/min
Temperature:25°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

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