Applications
25-OH-Vitamin D3 in Human Serum using Novum SLE and Kinetex 2.6um F5 100 x 4.6mm
22884
Vitamins
Clinical

Applications

25-OH-Vitamin D3 in Human Serum using Novum SLE and Kinetex 2.6um F5 100 x 4.6mm
Analytes
1
Compound Name
Calcifediol
CID
5283731
Molecular Formula
C27H44O2
Molecular Weight
400
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 22884)
Elution Type:
Gradient
Mobile Phase:
A: 0.1% FA in Water
B: 0.1%FA in MeOH
Gradient Profile:
Step No.Time(min)%A%B
101585
251585
35.010100
46.50100
56.511585
681585
Flow Rate:
0.75 mL/min
Temperature:
22°C
Detection:
Tandem Mass Spec (MS-MS) @ 22°C
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