Applications
25-OH-Vitamin D2 in Human Serum using Novum SLE and Kinetex 2.6um F5 100 x 4.6mm
22883
Separation Mode: Reversed Phase
Reversed Phase
F5
HPLC
Vitamins
Clinical

Applications

25-OH-Vitamin D2 in Human Serum using Novum SLE and Kinetex 2.6um F5 100 x 4.6mm

Analytes

1
Compound Name
25-Hydroxycalciferol
CID
5710148
Molecular Formula
C28H44O2
Molecular Weight
412
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
2

Conditions

LC Conditions (App ID: 22883)
Column:Kinetex 2.6 µm F5 100 Å, LC Column 100 x 4.6 mm, Ea
Brand Name:Kinetex
Part No:00D-4723-E0
Elution Type:Gradient
Mobile Phase:A: 0.1% FA in Water
B: 0.1%FA in MeOH
Gradient Profile:
Step No.Time(min)%A%B
101585
251585
35.010100
46.50100
56.511585
681585
Flow Rate:0.75 mL/min
Temperature:22°C
Detection:Tandem Mass Spec (MS-MS) @ 22°C

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