Applications
Acrylamide from Coffee by SLE and LC/MS/MS
22851
Separation Mode: Reversed Phase
Reversed Phase
Hydro-RP
HPLC

Applications

Acrylamide from Coffee by SLE and LC/MS/MS

Analytes

1
2
Compound Name
Acrylamide
CID
6579
Molecular Formula
C3H5NO
Molecular Weight
71
No. Hydrogen Bond Acceptors
1
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 22851)
Column:Synergi 4 µm Hydro-RP 80 Å, LC Column 50 x 2 mm, Ea
Brand Name:Synergi
Part No:00B-4375-B0
Elution Type:Gradient
Mobile Phase:A: 0.1% FA in Water
B: 0.1%FA in MeOH
Gradient Profile:
Step No.Time(min)%A%B
101000
20.61000
30.850100
430100
53.011000
651000
Flow Rate:0.3 mL/min
Temperature:22°C
Detection:Tandem Mass Spec (MS-MS) @ 22°C

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