Applications
Nicotines at High pH using Kinetex 5u EVO C18 100x3.0mm
22721
Separation Mode: Reversed Phase
Reversed Phase
EVO C18
HPLC
Nicotinic Agonists
Pharmaceutical/Biopharmaceutical
Clinical
Forensics/Toxicology
Therapeutic / Clinical
Therapeutic / Clinical
Therapeutic / Clinical

Applications

Nicotines at High pH using Kinetex 5u EVO C18 100x3.0mm

Analytes

1
2
3
4
5
6
Compound Name
Hydroxycotinine
CID
414
Molecular Formula
C10H12N2O2
Molecular Weight
192
No. Chiral Centers:
2
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 22721)
Column:Kinetex 5 µm EVO C18 100 Å, LC Column 100 x 3.0 mm, Ea
Brand Name:Kinetex
Part No:00D-4633-Y0
Elution Type:Gradient
Mobile Phase:A: 20 mM Ammonium bicarbonate pH 8.2
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
109010
231090
33.19010
459010
55955
Flow Rate:0.75 mL/min
Temperature:22°C
Detection:Mass Spectrometer (MS) amu @ 22°C

Order Items Used in This Application

Similar Applications
No Data