Applications
3-Hydroxycotinine using SPE with Gemini NX-C18, LC-MSMS (Fig.3d)
22039
Separation Mode: Reversed Phase
Reversed Phase
NX-C18
HPLC
Nicotinic Agonists

Applications

3-Hydroxycotinine using SPE with Gemini NX-C18, LC-MSMS (Fig.3d)

Analytes

1
2
3
4
5
Compound Name
Nornicotine
CID
412
Molecular Formula
C9H12N2
Molecular Weight
148
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 22039)
Column:Gemini 3 µm NX-C18 110 Å, LC Column 50 x 2 mm, Ea
Brand Name:Gemini
Part No:00B-4453-B0
Elution Type:Gradient
Mobile Phase:A: 20mM Ammonium Bicarbonate
B: 100% Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
109010
232575
33.19010
459010
Flow Rate:500 µL/min
Temperature:25°C
Detection:Tandem Mass Spec (MS-MS) @ 22°C

Sample Preparation Details
Description:Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:8B-S029-UBJ
Pre Treatment Note:Reconstitution Solvent: 500uL Acetonitrile/20mM Ammonium bicarbonate (10:90)
SPE Condition 1:2 mL Methanol at 1 mL/min
SPE Condition 2:2 mL Ammonium acetate buffer at 1 mL/min
SPE Load:Load 2.00 mL Sample diluted in 0.5 diluted urine sample
SPE Wash 1:2 mL Ammonium acetate buffer at 1 mL/min
SPE Wash 2:2 mL 30% Methanol at 1 mL/min
SPE Dry:> 10" Hg for 5 min to remove residual water
SPE Elution 1:2 mL 1.5% Ammonium hydroxide methanol solution at 1 mL/min
SPE Elution 2:2 mL 1.5% Ammonium hydroxide methanol solution at 1 mL/min
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:500 µL 500uL ACN/20mM Ammonium bicarbonate (10:90)

Order Items Used in This Application

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