Applications
EPA 539 on Gemini NX-C18 3 um 100 X 2.0 mm
21939
Estrogens

Applications

EPA 539 on Gemini NX-C18 3 um 100 X 2.0 mm
Analytes
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Compound Name
Estriol
CID
5756
Molecular Formula
C18H24O3
Molecular Weight
288
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 21939)
Elution Type:
Gradient
Mobile Phase:
A: 0.2%NH4OH in water
B: 0.2%NH4OH in MeOH
Gradient Profile:
Step No.Time(min)%A%B
106535
20.016535
30.63565
47.53565
58.51585
6131585
713.016535
8156535
Flow Rate:
0.2 mL/min
Temperature:
22°C
Detection:
Tandem Mass Spec (MS-MS) @ 22°C
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