Applications
Human Plasma TMP & Thiamine by Gemini-NX 3u C18 100x3mm with Impact Plate_2.5nmol
21875
Separation Mode: Reversed Phase
Reversed Phase
NX-C18
HPLC
Vitamin B Complex
Clinical

Applications

Human Plasma TMP & Thiamine by Gemini-NX 3u C18 100x3mm with Impact Plate_2.5nmol

Analytes

1
2
Compound Name
Trimethoprim
CID
5578
Molecular Formula
C14H18N4O3
Molecular Weight
290
No. Hydrogen Bond Acceptors
7
No. Hydrogen Bond Donors
2

Conditions

LC Conditions (App ID: 21875)
Column:Gemini 3 µm NX-C18 110 Å, LC Column 100 x 3 mm, Ea
Brand Name:Gemini
Part No:00D-4453-Y0
Elution Type:Gradient
Mobile Phase:A: 25nM Na2HPO4, 10% methanol
B: 25mM Na2HPO4, 70% methanol
Gradient Profile:
Step No.Time(min)%A%B
10397
20.252575
30.752575
433565
541000
651000
75.1397
88397
Flow Rate:0.75 mL/min
Temperature:22°C
Detection:Fluorescence (FLUOR) nm @ 22°C

Sample Preparation Details
Description:Impact Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk
Part Number:CE0-7565
SPE Load:Load Sample
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:0 µL initial mobile phase

Order Items Used in This Application

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