Applications
Blank Plasma Samples Extracted from Phree Showing Endogenous Level of Vitamin D3 Using Kinetex C18
21870
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC

Applications

Blank Plasma Samples Extracted from Phree Showing Endogenous Level of Vitamin D3 Using Kinetex C18

Analytes

Analytes not found

Conditions

LC Conditions (App ID: 21870)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 30 x 3 mm, Ea
Brand Name:Kinetex
Part No:00A-4462-Y0
Elution Type:Gradient
Mobile Phase:A: 0.1% formic acid/water
B: 0.1% Formic acid/methanol
Gradient Profile:
Step No.Time(min)%A%B
104060
20.5595
32595
42.014060
53.54060
Flow Rate:0.6 mL/min
Temperature:22°C
Detection:Mass Spectrometer (MS) amu @ 22°C

Sample Preparation Details
Description:Phree Phospholipid Removal, 30 mg / well, 96-Well Plates, 2/PK
Part Number:8E-S133-TGB
SPE Load:Load Sample
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:0 µL initial mobile phase

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