Applications
Extraction of Mono Hydroxy Vitamin D2 and D3 from Phree on Kinetex C18 2.6u 30x3 mm
21868
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Vitamins
Clinical

Applications

Extraction of Mono Hydroxy Vitamin D2 and D3 from Phree on Kinetex C18 2.6u 30x3 mm

Analytes

1
2
3

Analyte not found

Conditions

LC Conditions (App ID: 21868)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 30 x 3 mm, Ea
Brand Name:Kinetex
Part No:00A-4462-Y0
Elution Type:Gradient
Mobile Phase:A: 0.1% formic acid/water
B: 0.1% Formic acid/methanol
Gradient Profile:
Step No.Time(min)%A%B
104060
20.5595
32595
42.014060
53.54060
Flow Rate:0.6 mL/min
Temperature:22°C
Detection:Mass Spectrometer (MS) amu @ 22°C

Sample Preparation Details
Description:Phree Phospholipid Removal, 30 mg / well, 96-Well Plates, 2/PK
Part Number:8E-S133-TGB
SPE Load:Load Sample
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:0 µL initial mobile phase

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