Applications
Extraction of Mono Hydroxy Vitamin D2 and D3 from Phree on Kinetex C18 2.6u 30x3 mm
21868
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Vitamins
Clinical

Applications

Extraction of Mono Hydroxy Vitamin D2 and D3 from Phree on Kinetex C18 2.6u 30x3 mm
Analytes
1
2
3

Analyte not found

Details
LC Conditions (App ID: 21868)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% formic acid/water
B: 0.1% Formic acid/methanol
Gradient Profile:
Step No.Time(min)%A%B
104060
20.5595
32595
42.014060
53.54060
Flow Rate:
0.6 mL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Description:
Phree Phospholipid Removal, 30 mg / well, 96-Well Plates, 2/PK
Part Number:
SPE Load:
Load Sample
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
0 µL initial mobile phase

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