Applications
Human Plasma Vitamin C on Kinetex 5u XB-C18, 150x4.6mm (1.56ug/mL)_LOQ
21446
Separation Mode: Reversed Phase
Reversed Phase
XB-C18
HPLC
Antioxidants
Clinical

Applications

Human Plasma Vitamin C on Kinetex 5u XB-C18, 150x4.6mm (1.56ug/mL)_LOQ
Analytes
1
2
Compound Name
Ascorbic Acid
Details
LC Conditions (App ID: 21446)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% formic acid
B: Acetonitrile 100%
Gradient Profile:
Step No.Time(min)%A%B
101000
23.51000
33.60100
450100
55.11000
671000
Flow Rate:
800 µL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C
Sample Preparation Details
Description:
Impact Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk
Part Number:
SPE Load:
Load Sample
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
0 µL initial mobile phase

Order Items Used in This Application

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