Applications
Human Plasma Vitamin C on Kinetex 5u XB-C18, 150x4.6mm Wavelength Study
21442
Antioxidants
Clinical

Applications

Human Plasma Vitamin C on Kinetex 5u XB-C18, 150x4.6mm Wavelength Study
Analytes
1
Compound Name
Ascorbic Acid
CID
5785
Molecular Formula
C6H8O6
Molecular Weight
176
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
4
Details
LC Conditions (App ID: 21442)
Elution Type:
Gradient
Mobile Phase:
A: 0.1% formic acid
B: Acetonitrile 100%
Gradient Profile:
Step No.Time(min)%A%B
101000
23.51000
33.60100
450100
55.11000
671000
Flow Rate:
800 µL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C
Sample Preparation Details
SPE Load:
Load Sample
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
0 µL initial mobile phase