Applications
Automated Extraction of Tricyclic Antidepressants by Perkin Elmer Multiprobe II
20937
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antidepressive Agents, Tricyclic

Applications

Automated Extraction of Tricyclic Antidepressants by Perkin Elmer Multiprobe II
Analytes
1
2
3
4
5
6
7
8
9
10
11
12
13
14
Compound Name
Doxepin
Details
LC Conditions (App ID: 20937)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 0.1% formic acid/water
B: 0.1% Formic acid/methanol
Gradient Profile:
Step No.Time(min)%A%B
106040
23.52080
342080
44.016040
556040
Flow Rate:
0.4 mL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Description:
Strata-X-Drug B 33 µm Polymeric Strong Cation, 30 mg / well, 96-Well Plates, 2/Pk
Part Number:
Pre Treatment Note:
To do % absolute recovery, the IS was spiked in the initial mobile phase in the reconstitution step
SPE Load:
Load 0.50 mL Sample diluted in 100 mM sodium acetate buffer (pH5.0)
SPE Wash 1:
0.8 mL 100mM sodium acetate buffer (pH5.0) at 1 mL/min
SPE Wash 2:
0.8 mL Methanol at 1 mL/min
SPE Dry:
8-10 mins at 10" of Hg under N2 at 45 degC
SPE Elution 1:
0.4 mL Methanol/ACN(5/50) + 2% ammonium hydroxide at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
0.50 mL LC initial mobile phase A/B(60/40)

Order Items Used in This Application

Similar Applications
search
No Data