Applications
T3/T4 Plasma Extract by LC/MS/MS on Kinetex C18, 2.6 um, 75x2.1 mm
20929
Clinical

Applications

T3/T4 Plasma Extract by LC/MS/MS on Kinetex C18, 2.6 um, 75x2.1 mm
Analytes
1
2
3

Analyte not found

Details
LC Conditions (App ID: 20929)
Elution Type:
Gradient
Mobile Phase:
A: 0.05% Formic Acid in DI H2O
B: 50:50 MeOH:ACN
Gradient Profile:
Step No.Time(min)%A%B
107030
22.53070
333070
43.011090
541090
64.017030
75.57030
Flow Rate:
600 mL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) amu @ 0°C
Sample Preparation Details
SPE Condition 1:
1 mL 100% MeOh at 1 mL/min
SPE Condition 2:
1 mL 0.1 N HCl at 1 mL/min
SPE Sample PreTreatment:
Extraction Procedure: Activate with 1 mL MeOH Equilibrate the SPE cartridge with 1 mL 0.1 M HCl Load sample (0.4 mL serum/plasma + 1 mL 0.1 M HCl) Wash SPE with 1 mL 0.1 M HCl Wash SPE with 1 mL 100% MeOH Dry the SPE bed under maximum vacuum for 5 min Elute the analytes with 2x1 mL 2.5% NH4OH in MeOH Evaporate the solvent to dryness under gentle N2 stream at 40-45°C Reconstitute the dry residue in 200 uL initial MP (30%MeOH) solvent
SPE Load:
Load 0.10 mL Sample diluted in 0.1 1N HCl
SPE Wash 1:
1 mL 0.1 N HCl at 1 mL/min
SPE Wash 2:
1 mL 100% MeOH at 1 mL/min
SPE Dry:
5 min under max vac
SPE Elution 1:
1 mL 2.5% NH4OH in MeOH at 1 mL/min
SPE Elution 2:
1 mL 2.5% NH4OH in MeOH at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
200 µL 30% MeOH in 0.05% HCOOH
Sample Notes:
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