Applications
Extraction of Synthetic Cannabinoids in Human Urine Using Strata-X-Drug B
20925
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Forensics/Toxicology
Clinical
Toxicology / Drug Screening / TDM
Therapeutic / Clinical

Applications

Extraction of Synthetic Cannabinoids in Human Urine Using Strata-X-Drug B
Analytes
1
2
3
4
5
6
7
8
9
10
11
12

Analyte not found

Details
LC Conditions (App ID: 20925)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 10mM ammonium formate
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
105545
275050
37.01595
410595
Flow Rate:
0.6 mL/min
Temperature:
22°C
Detection:
Mass Spectrometer (MS) amu @ 22°C
Sample Preparation Details
Description:
Strata-X-Drug B 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:
Pre Treatment Note:
Extraction Protocol (No need for conditioning or equilibration of the cartridge) Load: Pre-treated samples from hydrolysis (as below) Sample Hydrolysis: Combine 1 mL Human Urine sample (spiked with analytes at 50 ng/mL), 2 mL of 100 mM sodium acetate buffer, pH 5.0, 25 uL -D-Glucoronidase (Patella Vulgata from Sigma, 100KU). Vortex 10-15 secs, followed by incubation for 2 hours in a shaker at 55o C to complete hydrolysis of the glucuronides. Wash 1: 2 mL 100 mM sodium acetate buffer, pH 5.0 Wash 2: 2 mL Acetonitrile: 100 mM sodium acetate buffer, pH 5.0 (30/70) Dry: 15 mins under full vacuum Elute: 2 ml Ethyl acetate:Isopropanol (85/15) Blow Down: To dryness under nitrogen at 40-45o C Reconstitute: With 500 uL of initial HPLC mobile phase (A/B=55/45) spiked with Internal standard at 100ng/mL
SPE Load:
Load 1.00 mL Sample diluted in 100 mM sodium acetate buffer, pH 5.0
SPE Wash 1:
2 mL 100 mM sodium acetate buffer, pH 5.0 at 1 mL/min
SPE Wash 2:
2 mL Acetonitrile: 100 mM sodium acetate buffer, pH 5.0 (30/70) at 1 mL/min
SPE Dry:
15 mins under 10-15" of Hg
SPE Elution 1:
2 mL Ethyl acetate:Isopropanol (85/15) at 1 mL/min
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
0.50 mL 500 uL of initial HPLC mobile phase (A/B=55/45)

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