Applications
Human Plasma Vitamin C on Kinetex 5u XB-C18, 150x4.6mm (Spiked)
20920
Separation Mode: Reversed Phase
Reversed Phase
XB-C18
HPLC
Antioxidants
Clinical

Applications

Human Plasma Vitamin C on Kinetex 5u XB-C18, 150x4.6mm (Spiked)

Analytes

1
Compound Name
Ascorbic Acid
CID
5785
Molecular Formula
C6H8O6
Molecular Weight
176
No. Hydrogen Bond Acceptors
6
No. Hydrogen Bond Donors
4

Conditions

LC Conditions (App ID: 20920)
Column:Kinetex 5 µm XB-C18 100 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Kinetex
Part No:00F-4605-E0
Elution Type:Gradient
Mobile Phase:A: 0.1% formic acid
B: Methanol 100%
Gradient Profile:
Step No.Time(min)%A%B
101000
23.51000
33.60100
450100
55.11000
671000
Flow Rate:800 µL/min
Temperature:22°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

Sample Preparation Details
Description:Impact Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk
Part Number:CE0-7565
SPE Load:Load Sample
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:0 µL initial mobile phase

Order Items Used in This Application

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