Applications
Nicotinic acid / Nicotinamide (10 ng/mL) in Human Plasma by Impact on Gemini 3µm C18 100x4.6mm
20906
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antilipemic Agents
Clinical
Clinical Research
Therapeutic / Clinical

Applications

Nicotinic acid / Nicotinamide (10 ng/mL) in Human Plasma by Impact on Gemini 3µm C18 100x4.6mm

Analytes

1
2
Compound Name
Nicotinamide
CID
936
Molecular Formula
C6H6N2O
Molecular Weight
122
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 20906)
Column:Gemini 3 µm C18 110 Å, LC Column 100 x 4.6 mm, Ea
Brand Name:Gemini
Part No:00D-4439-E0
Elution Type:Gradient
Mobile Phase:A: 0.1% formic acid
B: Methanol 100%
Gradient Profile:
Step No.Time(min)%A%B
109010
22.51090
32.69010
449010
Flow Rate:0.6 mL/min
Temperature:22°C
Detection:Electrospray Mass Spec (ESMS) @ 22°C

Sample Preparation Details
Description:Impact Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk
Part Number:CE0-7565
SPE Load:Load Sample
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:0 µL initial mobile phase

Order Items Used in This Application

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