Applications
Melatonin in commercial formuation on Kinetex 5u C18 50x4.6mm
20900
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Antioxidants
Food & Beverage

Applications

Melatonin in commercial formuation on Kinetex 5u C18 50x4.6mm

Analytes

1
2
Compound Name
Melatonin
CID
896
Molecular Formula
C13H16N2O2
Molecular Weight
232
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
2

Conditions

LC Conditions (App ID: 20900)
Column:Kinetex 5 µm C18 100 Å, LC Column 50 x 4.6 mm, Ea
Brand Name:Kinetex
Part No:00B-4601-E0
Elution Type:Gradient
Mobile Phase:A: 0.25% Phosphoric Acid in MilliQ Water
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
109010
273070
381090
4101090
510.19010
6139010
Flow Rate:1.5 mL/min
Temperature:30°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

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Similar Applications
Kinetex 5 µm C18 100 Å, LC Column 50 x 4.6 mm, Ea
Reversed Phase