Applications
TMP and Thiamine Multi-Standard by Impact PPT on Kinetex 5u C18 100x4.6mm
20882
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Vitamin B Complex
Clinical
Clinical Research
Therapeutic / Clinical

Applications

TMP and Thiamine Multi-Standard by Impact PPT on Kinetex 5u C18 100x4.6mm

Analytes

1
2
Compound Name
Monofosfotiamina
CID
10761
Molecular Formula
C12H18ClN4O4PS
Molecular Weight
380
No. Hydrogen Bond Acceptors
8
No. Hydrogen Bond Donors
3

Conditions

LC Conditions (App ID: 20882)
Column:Kinetex 5 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea
Brand Name:Kinetex
Part No:00D-4601-E0
Elution Type:Gradient
Mobile Phase:A: 25mM Na2HPO4, 10% Methanol, pH 7.0
B: 25mM Na2HPO4, 70% Methanol, pH 7.0
Gradient Profile:
Step No.Time(min)%A%B
10973
20.257525
30.757525
436535
544060
64.5973
75973
Flow Rate:1 mL/min
Temperature:25°C
Detection:Fluorescence (FLUOR) nm @ 22°C

Sample Preparation Details
Description:Impact Protein Precipitation, 2mL Square Well Filter Plate, 2/Pk
Part Number:CE0-7565
Pre Treatment Note:Add 400µL of methanol to a well of a Protein Precipitation Plate; Add 100µL plasma to the well. Mix 3 times with pipette tip (or vortex the whole plate briefly); Wait for 5 minutes; Filter through the Protein Precipitation Plate under vacuum (5 Hg) for 5 minutes. Transfer 50 µL to an autosampler vial (or a well of 96-well plate). Add 100µL water and 25 µL of derivatizing reagent*. Cover the vial with a lid. Vortex for 15 seconds. Put the vial into an autosampler. All conditions are at room temperature (RT). It is ready to inject. *Derivatizing reagent: Potassium ferricyanide [K3Fe(CN)6] was prepared as the following: dissolve 6mg of K3Fe(CN)6 in 15mL 15% sodium hydroxide solution. Prepare fresh daily.
SPE Load:Load Sample
SPE Evaporation:Evaporate to dryness

Order Items Used in This Application

Similar Applications
Kinetex 5 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea
Reversed Phase
Kinetex 5 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea
Reversed Phase