Applications
OPA Derivitization of Tyramine and Histamine using Kinetex 2.6u C18
20873
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Sympathomimetics
Food & Beverage

Applications

OPA Derivitization of Tyramine and Histamine using Kinetex 2.6u C18
Analytes
1
2
Compound Name
Histamine
Details
LC Conditions (App ID: 20873)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 20mM Sodium Acetate pH 7.2 w/0.03% Sodium Azide (pre-filtered 0.2um)
B: 50:50 Acetonitrile/Methanol
Gradient Profile:
Step No.Time(min)%A%B
10973
2202080
320.5973
423973
Flow Rate:
1.2 mL/min
Temperature:
30°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

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