Applications
OPA Derivitization in Wine of Histadine and Histamine using Kinetex 2.6u C18
20869
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Histamine Agents
Food & Beverage

Applications

OPA Derivitization in Wine of Histadine and Histamine using Kinetex 2.6u C18

Analytes

1
2
Compound Name
Histidine
CID
6274
Molecular Formula
C6H9N3O2
Molecular Weight
155
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
3

Conditions

LC Conditions (App ID: 20869)
Column:Kinetex 2.6 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea
Brand Name:Kinetex
Part No:00D-4462-E0
Elution Type:Gradient
Mobile Phase:A: 20mM Sodium Acetate pH 7.2 w/0.03% Sodium Azide (pre-filtered 0.2um)
B: 50:50 Acetonitrile/Methanol
Gradient Profile:
Step No.Time(min)%A%B
10973
2202080
320.5973
423973
Flow Rate:1.2 mL/min
Temperature:30°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

Order Items Used in This Application

Similar Applications
Kinetex 2.6 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea
Reversed Phase