Applications
Testosterone-Oxime on Gemini-NX C18, 3um, 50x2 mm
20844
Separation Mode: Reversed Phase
Reversed Phase
NX-C18
HPLC
Androgens
Clinical
Clinical Research
Therapeutic / Clinical

Applications

Testosterone-Oxime on Gemini-NX C18, 3um, 50x2 mm

Analytes

1
2
3
Compound Name
Testosterone
CID
6013
Molecular Formula
C19H28O2
Molecular Weight
288
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 20844)
Column:Gemini 3 µm NX-C18 110 Å, LC Column 50 x 2 mm, Ea
Brand Name:Gemini
Part No:00B-4453-B0
Elution Type:Gradient
Mobile Phase:A: 0.1% Formic Acid plus 1 mM Ammonium Formate in DI H2O
B: 0.1% Formic Acid plus 1 mM Ammonium Formate in ACN
Gradient Profile:
Step No.Time(min)%A%B
107030
21.51090
32.51090
42.67030
557030
Flow Rate:0.4 mL/min
Temperature:50°C
Detection:Mass Spectrometer (MS) amu @ 0°C

Sample Preparation Details
Description:Strata-X-A 33 µm Polymeric Strong Anion, 30 mg / 3 mL, Tubes, 50/Pk
Part Number:8B-S123-TBJ
SPE Condition 1:1 mL 100% MeOH at 1 mL/min
SPE Condition 2:1 mL DI H2O at 1 mL/min
SPE Load:Load 0.25 mL Sample diluted in 1 mL DI H2O
SPE Wash 1:0.6 mL 50% MeOH at 1 mL/min
SPE Elution 1:0.6 mL 100% MeOH at 1 mL/min
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:0 µL initial mobile phase

Order Items Used in This Application

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