Applications
Heterocyclic Amines by LCMS using Kinetex 5u C18 50x2.1mm
20771
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Neurotoxins
Environmental
Food & Beverage
Environmental / Health
Product / Trade Good
Environmental / Health

Applications

Heterocyclic Amines by LCMS using Kinetex 5u C18 50x2.1mm
Analytes
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Analyte not found

Details
LC Conditions (App ID: 20771)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 30 mM Ammonium formate
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
10955
254060
35.10100
460100
57955
Flow Rate:
500 µL/min
Temperature:
22°C
Detection:
Tandem Mass Spec (MS-MS) @ 22°C
Sample Preparation Details
Description:
Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:
SPE Condition 1:
3 mL Methanol at 1 mL/min
SPE Condition 2:
4 mL Water
SPE Load:
Load Sample
SPE Wash 1:
3 mL 0.1M HCl
SPE Wash 2:
3 mL Methanol
SPE Elution 1:
3 mL 0.2M Ammonia Solution made up in methanol
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
0 µL Mobile phase

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