Applications
Biogenic Amines by LCMS using Kinetex 1.7u C18 50x3.0mm
20685
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Mutagens
Clinical
Biochemical Compound / Nutrient
Therapeutic / Clinical

Applications

Biogenic Amines by LCMS using Kinetex 1.7u C18 50x3.0mm
Analytes
1
2
3
4
5
6
7
8
9
10
11
12
Compound Name
Molport-003-844-559
CID
105041
Molecular Formula
C10H9N5
Molecular Weight
199
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
1
Details
LC Conditions (App ID: 20685)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 30mM Ammonium formate
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
108020
234060
33.10100
440100
54.18020
658020
Flow Rate:
600 µL/min
Temperature:
22°C
Detection:
Tandem Mass Spec (MS-MS) @ 22°C
Sample Preparation Details
Description:
Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:
SPE Condition 1:
3 mL Methanol at 1 mL/min
SPE Condition 2:
4 mL Water
SPE Load:
Load Sample
SPE Wash 1:
3 mL 0.1M HCl
SPE Wash 2:
3 mL Methanol
SPE Elution 1:
3 mL 0.2M Ammonia Solution made up in methanol
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
0 µL Mobile phase

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