Applications
Testosterone and 17-OH-Progesterone by LCMS using Gemini NX 3u C18 50x2.1mm
20652
Separation Mode: Reversed Phase
Reversed Phase
NX-C18
HPLC
Androgens
Clinical
Biochemical Compound / Nutrient
Therapeutic / Clinical
Chemical Compound
Therapeutic / Clinical
Therapeutic / Clinical

Applications

Testosterone and 17-OH-Progesterone by LCMS using Gemini NX 3u C18 50x2.1mm

Analytes

1
2
Compound Name
Testosterone
CID
6013
Molecular Formula
C19H28O2
Molecular Weight
288
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 20652)
Column:Gemini 3 µm NX-C18 110 Å, LC Column 50 x 2 mm, Ea
Brand Name:Gemini
Part No:00B-4453-B0
Elution Type:Gradient
Mobile Phase:A: Water with 0.1% formic acid
B: Acetonitrile with 0.1% formic acid
Gradient Profile:
Step No.Time(min)%A%B
108020
22.52575
34.52575
44.68020
568020
Flow Rate:0.4 mL/min
Temperature:22°C
Detection:Tandem Mass Spec (MS-MS) @ 22°C

Sample Preparation Details
Description:Strata-X-A 33 µm Polymeric Strong Anion, 30 mg / 1 mL, Tubes, 100/Pk
Part Number:8B-S123-TAK
SPE Condition 1:1 mL Methanol
SPE Condition 2:1 mL Water
SPE Load:Load Sample
SPE Wash 1:0.6 mL 50% MeOH
SPE Dry:high vacuum for 2-3 min
SPE Elution 1:0.6 mL 100% MeOH
SPE Elution 2:0.6 mL 100% MeOH
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:0 µL initial mobile phase

Order Items Used in This Application

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