Applications
Nicotine and metabolites by LCMS using Gemini NX 3u C18 100x2.1mm
20651
Separation Mode: Reversed Phase
Reversed Phase
NX-C18
HPLC
Nicotinic Agonists
Clinical
Therapeutic / Clinical
Biochemical Compound / Nutrient
Therapeutic / Clinical

Applications

Nicotine and metabolites by LCMS using Gemini NX 3u C18 100x2.1mm

Analytes

1
2
3
4
5
Compound Name
Nornicotine
CID
412
Molecular Formula
C9H12N2
Molecular Weight
148
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 20651)
Column:Gemini 3 µm NX-C18 110 Å, LC Column 100 x 2 mm, Ea
Brand Name:Gemini
Part No:00D-4453-B0
Elution Type:Gradient
Mobile Phase:A: 20mM Ammonium Bicarbonate
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
109010
232575
33.19010
459010
Flow Rate:0.5 mL/min
Temperature:22°C
Detection:Tandem Mass Spec (MS-MS) @ 22°C

Sample Preparation Details
Description:Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk
Part Number:8B-S029-UBJ
SPE Condition 1:2 mL Methanol
SPE Condition 2:2 mL Ammonium acetate buffer
SPE Load:Load Sample
SPE Wash 1:2 mL Ammonium acetate buffer
SPE Wash 2:2 mL 30% Methanol
SPE Dry:10" Hg for 5 min
SPE Elution 1:2 mL 1.5% Ammonium hydroxide methanol solution
SPE Elution 2:2 mL 1.5% Ammonium hydroxide methanol solution
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:0 µL initial mobile phase

Order Items Used in This Application

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