Applications
Aldosterone analysis by LCMS using Kinetex 2.6um XB-C18 50x3.0mm
20648
Clinical
Clinical Research
Therapeutic / Clinical

Applications

Aldosterone analysis by LCMS using Kinetex 2.6um XB-C18 50x3.0mm
Analytes
1
Compound Name
Aldosterone
CID
5839
Molecular Formula
C21H28O5
Molecular Weight
360
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
2
Details
LC Conditions (App ID: 20648)
Elution Type:
Gradient
Mobile Phase:
A: Water
B: 50/50 AcCN/Methanol
Gradient Profile:
Step No.Time(min)%A%B
107030
241090
351090
45.17030
577030
Flow Rate:
0.5 mL/min
Temperature:
22°C
Detection:
Tandem Mass Spec (MS-MS) @ 22°C
Sample Preparation Details
SPE Condition 1:
3 mL Methanol
SPE Condition 2:
3 mL 25 mM Ammonium Bicarbonate
SPE Load:
Load 0.50 mL Sample diluted in 25 mM Ammonium Bicarbonate
SPE Wash 1:
3 mL 25mm ammonium bicarbonate buffer
SPE Wash 2:
3 mL 25% methanol in water
SPE Dry:
5 min at high vacuum
SPE Elution 1:
1 mL Ethyl Acetate:Isopropanol:NH4OH (7:2:1)
SPE Elution 2:
1 mL Ethyl Acetate:Isopropanol:NH4OH (7:2:1)
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
0 µL initial mobile phase
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